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Bachelor/Master thesis: Enzymatic Immobilization of Proteins
The immobilization of proteins to material surfaces confers novel functionalities, e.g. catalytic activity, biocompatibility, or antifouling properties. In this work, Protein immobilization will be achieved using crosslinking enzymes.
Keywords: protein immobilization, enzymes, material surface, biochemistry, molecular biology
The student will have the unique opportunity of working with various proteins and enzymes belonging to different classes, e.g. oxidoreductases, hydrolases, and fluorescent proteins, and become familiar with many techniques from biochemistry, molecular biology, and materials science.
Expression and purification of recombinant proteins, e.g. affinity chromatography; Protein characteriza-tion, e.g. SDS PAGE, UV-Vis, fluorescence, activity measurements, stability assessment; Characterization of functionalized materials, e.g. microscopy, flow cytometry; Image analysis, e.g. Jalview for SDS PAGE analyses; Buffers and media preparation.
Travel compensation available.
The student will have the unique opportunity of working with various proteins and enzymes belonging to different classes, e.g. oxidoreductases, hydrolases, and fluorescent proteins, and become familiar with many techniques from biochemistry, molecular biology, and materials science. Expression and purification of recombinant proteins, e.g. affinity chromatography; Protein characteriza-tion, e.g. SDS PAGE, UV-Vis, fluorescence, activity measurements, stability assessment; Characterization of functionalized materials, e.g. microscopy, flow cytometry; Image analysis, e.g. Jalview for SDS PAGE analyses; Buffers and media preparation.
Travel compensation available.
The final aim is evaluating the effect of the enzymatic crosslinking with tyrosinase/sortase on the cata-lytic activity of proteins. Moreover, the work will focus on characterizing and optimising the conditions for crosslinking.
The final aim is evaluating the effect of the enzymatic crosslinking with tyrosinase/sortase on the cata-lytic activity of proteins. Moreover, the work will focus on characterizing and optimising the conditions for crosslinking.
Dr. Greta Faccio
greta.faccio @ empa.ch
ResearcherID B-4815-2013
Dr. Greta Faccio greta.faccio @ empa.ch ResearcherID B-4815-2013